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KMID : 0350519930460031137
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Journal of Catholic Medical College
1993 Volume.46 No. 3 p.1137 ~ p.1145
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Ultrastructural Observations of Human Peripheral Blood Mononuclear Cells After Exposure to Magnetic Resonance
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Abstract
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MR M(agnetic Resonance) imaging provides information of not only anatomic but also physiologic characteristics of tissues and does not use ionizing radiation. The use of MR as a diagnostic procedure has increased rapidly in recent years. Studies
of
MR-related biologic effects have been predominantly negative to date. On the other hand, some several studies, while controversial, have suggested potential adverse biologic effects from MR.
To determine whether 0.5 Tesla MR produces any morphologic changes on the cell surface and intracellular ultrastructure, we examined healthy human peripheral blood mononuclear cells(PBMC) by scanning electron microscopy(SEM) and transmission
electron
microscopy(TEM). Exposure to MR was carried out at 0.5 Tesla static magnetic field, 7 mT/m gradient magnetic field, 21.3 MHz radiofrequency with various pulse sequences. The protocol was varied somewhat for each experiments ; Repetition Time(TR)
were
2000 msec and 4000 msec, exposure times varying from 30 minutes ot 3 hours. The cells were fixed at 30 minutes, 1, 3, 5, 7 days after MR exposure. No significant cell surface changes of human PBMC from 30 minutes to 7 days after MR exposure were
noticed
on the SEM. Under TEM there was no differences in the appearance of nucleus, and intracellular microorganelles between the control and MR exposed PBMC. Cultured cells on SEM and TEM did not show any difference between various exposure parameters.
This morphological study demonstrated that short-term exposure to 0.5 Tesla MR did not cause significant cellular damage on human lymphocytes and monocytes in vitro. It therefore suggest that 0.5 Tesla MR as presently employed is free from
cellular
injury on human PBMC.
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